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. 2017 Jul 25;18(8):1605. doi: 10.3390/ijms18081605

Table 3.

Primers designed for the amplifications of the C. hamatus and D. labrax catalytic proteasome subunits cDNAs.

Primer Sequence Tm (°C)
Chbetalfor 5′-CCATATTGCAGTGATACAGCGAG-3′ 60.5
Chbetalrev 5′-CTCAGTCCTTCCTCAGGGG-3′ 60.1
Chbeta2for 5′-GTCGGGATACAGGGACCG-3′ 60.8
Chbeta2rev 5′-AGCGGTCACTTGGCGC-3′ 62.8
Chbeta5for 5′-GGGAGTTTCAAAGATGGCTCT-3′ 59.2
Chbeta5rev 5′-TTGTACTGCTGGTGCAGCAT-3′ 61.7
Dlbetalfor 5′-ATGATTTCTGCTCATGGTTTCGG-3′ 60.6
Dlbetalrev 5′-CCCTCCTCAGTCCTTCCTC-3′ 59.8
Dlbeta2for 5′-GCACCATGGAGTATTTAATCGGG-3′ 60.4
Dlbeta2rev 5′-GTGAGCGGTCACTTGGC-3′ 60.4
Dlbeta5for 5′-ATGGCTCTTGCTAGTGTGTTG-3′ 59.8
Dlbeta5rev 5′-CATGACTATGCCTGATCTTTGTACTG-3′ 60.3