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. 2017 Jul 25;18(8):1610. doi: 10.3390/ijms18081610

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Size distribution of EVs detected in conditioned media collected at 4 h from cells after microbubbles-assisted ultrasound (USMB, A), ultrasound (US, B), or microbubbles (MB, C) treatments, and from untreated cells (D). Surface area of EVs was measured using Image J [20] to calculate the apparent diameter of EV (nm). Bin size is 50 nm. EVs were detected from 10 images per grid (60,000× magnification) derived from four independent experiments. Percentage of EVs represents the percentage of mean of EVs at certain size range + standard deviation.