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. 2017 Jul 25;18(8):1610. doi: 10.3390/ijms18081610

Figure 5.

Figure 5

Western blotting on total cell and EV lysates prepared from FaDu cells and their supernatant collected at 4 h after microbubbles-assisted ultrasound (USMB). For western blotting, equal protein amounts of cell and EV lysates were loaded (4.5 µg of total proteins). Detection of the proteins was by using antibodies alix, tsg101, CD9, CD63, calnexin, and actin. Presence of alix (95 kDa), tsg101 (49 kDa), and CD9 (25 kDa) are shown (A). CD63 is shown as a broad band between 30–65 kDa (B). Calnexin (95 kDa) and actin (42 kDa) are present in both cell and EV lysates (C).