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. 2017 Jul 25;18(8):1614. doi: 10.3390/ijms18081614

Figure 2.

Figure 2

(A) Representative images of Wound Healing assay on HUVEC cells treated or not with sodium mesoglycan and Prisma® Skin 0.3 mg/mL. Bar: 100µm; (B) Results of Wound Healing assay analysis. The migration rate of individual cells was determined by measuring the distances covered from the initial time to the selected time-points (bar of distance tool, Leica ASF software). The data represent a mean of three independent experiments ± SEM, their statistical significance was evaluated using Student’s t-test, assuming a two-tailed distribution and unequal variance. ** p < 0.01; *** p < 0.001; (C) Representative images of analyzed fields of invasion assay; (D) Analysis of invasion speed of HUVEC cells with mesoglycan and Prisma® Skin. Data represent the mean cell counts of 12 separate fields per well ± SEM of five experiments with similar results. Bar: 50µm. ** p < 0.01; *** p < 0.001.