Figure 6.

(A) NO (nitric oxide) release detected as [NO₂⁻] µM by Griess reaction in presence of LPS (10 µg/mL) for 24 h, with or without L-Name (100 µM), mesoglycan and Prisma^® Skin 0.3 mg/mL; (B) HUVEC cells were treated with LPS (10 µg/mL) for 24 h and co-exposed or not with sodium mesoglycan and Prisma^® Skin 0.3 mg/mL and nuclear translocation of NF-κB p65 subunit was detected using immunofluorescence assay at confocal microscopy. Nuclei were stained with DAPI. Magnification 63× 1.4 NA. Bar = 10 µm. All the results are representative of 3 experiments with similar results; (C) NO release detected on SC cells in presence of LPS (10 µg/mL) for 24 h, with or without L-Name (100 µM), mesoglycan and Prisma^® Skin 0.3 mg/mL. As for HUVEC cells, data derive from the mean of three independent experiments showing similar results and have been analyzed using Student’s t-test, assuming a two-tailed distribution and unequal variance. * p < 0.05; ** p < 0.01; *** p < 0.001 for all treatments vs. untreated cells. ^§§ p < 0.01; ^§§§ p < 0.001 for all treatments vs. LPS-treated cells; (D) TNF-α production was measured in the supernatants of SC cells by ELISA kit. Cells were treated with LPS (10 µg/mL) alone or in combination with mesoglycan and Prisma^® Skin 0.3 mg/mL for 24 h. Data represent the mean of three independent experiments with similar results and analyzed by Student’s t-test, assuming a two-tailed distribution and unequal variance. *** p < 0.001.