Figure 1.

Tan-IIA inhibited migratory and invasive ability in human BCa cells. (A) Human BCa cells were treated with 0.2% DMSO as a vehicle control or 4 μg/mL Tan-IIA for 24 h and then seeded onto the transwell hanging insert for migration (24 h) and invasion (48 h) assays. Images were captured using an inverted microscope with 200× magnification; Scale bar: 50 μm. The migration and invasion of BCa cells were quantified by counting the stained cells that migrated into the underside of the hanging insert membrane; (B) human BCa cells were treated with different concentrations of Tan-IIA (1, 2 and 4 μg/mL) for 48 h. The protein of total cell lysates were then used to detect MMP-9/-2 protein expression using western blot, and the (C) supernatant was used to detect the enzymatic activity using zymography analysis. M: marker. Data are presented as means ± S.D. from three different experiments. ** p < 0.01 versus vehicle.