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. 2017 Jul 25;18(8):1616. doi: 10.3390/ijms18081616

Figure 3.

Figure 3

Tan-IIA inhibited the CCL2 expression and reversed the EMT in human BCa cells. (A) Human BCa cells were treated with increasing concentrations of Tan-IIA for 24 h. The expression of CCL2 was detected by PCR and qRT-PCR; (B) Human BCa cells were treated with increasing concentrations of Tan-IIA for 48 h. The supernatant was collected for CCL2 protein detection using ELISA assay; (C) BFTC cells were treated with or without 4 μg/mL Tan-IIA in the presence or absence of CCL2 recombinant protein for 48 h. The EMT-related gene expression was detected by western blot; (D) BFTC cells were treated with or without 4 μg/mL Tan-IIA in the presence or absence of 100 ng/mL human CCL2 recombinant protein for 24 h, followed by migration (24 h) or invasion (48 h) assays and analyzed as previous described. Data are presented as means ± S.D. from three different experiments. *** p < 0.001 versus vehicle.