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. 2017 Jul 26;18(8):1619. doi: 10.3390/ijms18081619

Figure 3.

Figure 3

(A) HeLa cells were pre-incubated of mollugin (20, 40, and 80 μM) for 12 h prior to stimulation with 10 ng/mL TNF-α for another 12 h. Whole cell extracts were analyzed by Western blot assay for COX-2, Cyclin D1, Bcl-2, cIAP-1, survivin, MMP-9, ICAM-1, and tubulin; (B) HeLa cells were pre-incubated of mollugin (20, 40, and 80 μM) for 12 h prior to stimulation with 10 ng/mL TNF-α for another 12 h. Total RNA was analyzed by RT-PCR for Cyclin D1, c-Myc, and VEGF. GAPDH was used as an equal loading control.