Figure 6.

Effect of mollugin on human xenograft model. (A,B) HeLa cells were implanted s.c. in the left flanks of nude mice, which were administered by gavage three times a week with vehicle (n = 5), mollugin (25 mg/kg, n = 5) or mollugin (75 mg/kg, n = 5) starting from day ten. Tumor volume and mouse weight were measured using calipers and a disital balance, respectively, every three days; (C) representative tumor masses of three groups, which were harvested 4 h after the last treatment (and the isolated tumors were photographed); (D) western blot analysis of p-p65 and COX-2 in tumor blocks is shown. Tubulin was used as a loading control.