Table 2.
Identification of salt stress regulated proteins through MALDI TOF/TOF MS analysis.
| Spot Number a | Accession Number | Homology Protein | Species | Putative Function | kDa | pI | Score | Coverage (%) | Fold Change b |
|---|---|---|---|---|---|---|---|---|---|
| Enhanced | |||||||||
| 1 | WP_006612358 | Carbon monoxide dehydrogenase | Bradyrhizobium sp. ORS 375 | Carbon metabolism | 15.40 | 6.66 | 89.4 | 43.92% | 6.26 ± 0.29 |
| 2 | WP_007615200 | Superoxide dismutase | Bradyrhizobium sp. WSM471 | Stress response | 22.63 | 7.02 | 230 | 68.18% | 4.79 ± 0.14 |
| 3 | WP_006609834 | ABC transporter | Bradyrhizobium sp. ORS 285 | Transporter | 30.81 | 10.01 | 73.7 | 43.10% | 4.99 ± 0.21 |
| 4 | WP_008542552 | DNA-binding response regulator | Bradyrhizobium japonicum USDA 6 | Signal transduction | 26.65 | 5.94 | 138 | 42.92% | Induced |
| Repressed | |||||||||
| 5 | WP_014491960 | Zinc protease | Bradyrhizobium japonicum USDA 6 | Protein metabolism | 50.66 | 5.54 | 207 | 28.29% | 1.87 ± 0.18 |
| 6 | WP_014491960 | Zinc protease | Bradyrhizobium japonicum USDA 6 | Protein metabolism | 50.66 | 5.54 | 222 | 28.73% | Supressed |
| 7 | WP_011087628 | Elongation factor Ts | Bradyrhizobium diazoefficiens JCM 10833 | Protein metabolism | 32.17 | 6.54 | 111 | 43.65% | 2.88 ± 0.23 |
| 8 | WP_011926260 | 30S ribosomal protein S6 | Bradyrhizobium sp. ORS278 | Protein metabolism | 17.41 | 5.01 | 124 | 58.17% | 3.91 ± 0.18 |
| 9 | WP_011083272 | ATP synthase subunit beta | Bradyrhizobium diazoefficiens JCM 10833 | Energy metabolism | 50.99 | 4.91 | 383 | 59.54% | 4.41 ± 0.21 |
| 10 | WP_007590612 | Enolase | Bradyrhizobium sp. WSM1253 | Energy metabolism | 45.36 | 4.84 | 271 | 34.19% | 1.77 ± 0.25 |
| 11 | WP_028155417 | β-ketoacyl-ACP synthase I | Bradyrhizobium japonicum USDA 6 | Other metabolism | 42.82 | 6.17 | 79.5 | 24.07% | 1.54 ± 0.1 |
| 12 | WP_014496130 | Demethylmenaquinone methyltransferase | Bradyrhizobium japonicum USDA 6 | Other metabolism | 25.90 | 5.89 | 383 | 41.00% | 1.54 ± 0.14 |
| 13 | WP_011090572 | Branched-chain amino acid ABC transporter substrate-binding protein |
Bradyrhizobium diazoefficiens JCM 10833 | Transporter | 40.84 | 7.77 | 151 | 36.03% | 3.80 ± 0.00 |
| 14 | WP_011088132 | DNA-directed RNA polymerease subnit alpha | Bradyrhizobium diazoefficiens JCM 10833 | Signal transduction | 38.03 | 4.63 | 306 | 44.61% | 1.82 ± 0.13 |
a Spot number corresponds to the spot number in Figure 5. b Data in the column represent the mean of three biological replicates with standard error. An individual flask was considered one biological replicate.