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. 2017 Jul 27;18(8):1630. doi: 10.3390/ijms18081630

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of Phosphoglycerate Kinase 1 (PGK1) on the proliferation of Hepatocellular Carcinoma (HCC) cells. (A) Endogenous expression of PGK1 in nomal and five HCC cell lines by Western blotting. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as internal control. (B) Transfection efficiency of PGK1 overexpress and knockdown in HCC. GAPDH was used as internal control. (C) Effect of PGK1 on proliferation of SNU182 and JHH5 cells by cell counting kit-8 (CCK8) assay. (D) Effect of PGK1 knockdown on proliferation of SNU449 and HCCLM3 cells by CCK8 assay. (E) Effect of PGK1 on proliferation of SNU182 and JHH5 cells by plate colony formation assay. (F) Effect of PGK1 knockdown on proliferation of SNU449 and HCCLM3 cells by plate colony formation assay. ** p < 0.01. NC, negative control; sh-PGK1, short hairpin RNA inhibiting PGK1 gene.