Figure 3.

Drug screening and therapeutic drug monitoring by Bioluminescence imaging (BLI). (A) Drug screening by BLI. Stable luciferase (FLuc)-transfected cancer cells can be used for high throughput screening (HTS). FLuc activity of cancer cells treated with 5 µM tyrosine kinase inhibitors was analyzed by BLI imaging after 24 h. Compounds that decrease FLuc activity were selected and used further in target study and preclinical drug efficiency; (B) Therapeutic drug monitoring by BLI imaging. Stable FLuc-transfected cancer cell was injected into the subcutaneous tumor (Xenograft) and then therapeutic effect was monitored. Decrease in FLuc activity was observed in the drug-treated mouse after 2 weeks of treatment.