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. 2017 Jul 28;18(8):1643. doi: 10.3390/ijms18081643

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Flow cytometric analysis of CD25 and CD69 surface expression on DHG-treated PBMC. PBMC from healthy donors (n = 7) were stimulated with PHA (1.5%) in the presence and in the absence of DHG. Unstimulated cells are included as control (PBMC) in the figure. Following 24 h of activation, CD3+/CD56− (black bars) and CD3−/CD56+ (gray bars) populations were analyzed for CD25+ expression (A) and CD69+ expression (B) and compared by ANOVA (* p < 0.05, compared with untreated PHA-activated cells). Bar graphs report mean values ± SD.