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. 2017 Jul 31;18(8):1656. doi: 10.3390/ijms18081656

Figure 8.

Figure 8

The effect of Nrf2 knockout on activation of NF-κB of the small intestine after exposure to abdominal irradiation. (A) Immunohistochemical analysis for NF-κB p65-stained sections of the small intestine. Small intestines of Nrf2+/+ mice and Nrf2−/− mice were harvested at 1, 3, and 6 h post-abdominal irradiation for immunohistochemical analysis. Scale bar, 100 μm; (B–D) The expression of NF-κB p65 in the cytoplasmic and nuclear lysates. Small intestines of mice were harvested at 6 h post-abdominal irradiation. The crypt cells were isolated from the small intestines to extract the cytoplasmic and nuclear lysates respectively. The cytoplasmic protein was identified by an anti-GAPDH antibody, and the nuclear protein was identified by an anti-LaminB1 antibody; (E) The mRNA levels of NF-κB target genes Bcl-2, uPA, and Xiap. Small intestines of mice were harvested at one day post-abdominal irradiation and total RNA was extracted to performed RT-PCR reaction. The data were expressed as the mean ± SD. ** p < 0.01, Nrf2−/− group versus the Nrf2+/+ group.