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. 2017 Aug 1;18(8):1661. doi: 10.3390/ijms18081661

Figure 7.

Figure 7

Site-directed mutagenesis analysis of ChiIV3 promoter in response to P. capsici inoculation. (A) The W-box locations in ChiIV3 promoter. The positions of the deletions are indicated by red numbers, and each W-box is indicated by a white box. The underlined nucleotides represent the core sequence of W-box; (B–D) The schematic diagram of pChiIV3:GUS and its W-box-mutant constructs (pChiIV3-W3m:GUS, pChiIV3-W4m:GUS, or pChiIV3-W5m:GUS, the mutated nucleotides of W-box are in red color) and the activities of GUS driven by pChiIV3:GUS and its three W-box-mutant in response to P. capsici inoculation in pepper leaves tissues transiently transformed with pChiIV3:GUS or its W-box-mutant constructs, respectively. The GV3101 cells containing the pChiIV3:GUS or its W-box-mutant constructs were vacuum-infiltrated into the pepper leaves and maintained in the greenhouse for 24 h. The infiltrated plants were then treated with P. capsici spores. The P. capsici-inoculated leaf tissues were harvested for GUS activity measurements at 2 dpi. Values are means ± SD (n = 6). Error bars indicate SD. Different letters indicate significant differences determined by Fisher’s protected LSD test (p < 0.05).