Figure 2.

Expression of IL-37 in hematopoietic cells reduces circulating immune cells. Plasma soluble E-selectin (sE-selectin) levels in Ldlr⁻/⁻ mice transplanted with either IL-37tg or wild-type (WT) bone marrow were determined just before Western-type diet (WTD)-feeding on regular chow diet, and after eight weeks of WTD-feeding (a); The indicated circulating immune cells in whole blood were determined by flow cytometry before (chow) and after three weeks of WTD-feeding (WTD) (b–k); Moreover, the ratio Ly6C^lo/Ly6C^hi was calculated (l); Data are expressed as means ± SEM; n = 14–15 per group; In panel 2a the effect of diet was determined using two-way ANOVA; * p < 0.05; ** p < 0.01; *** p < 0.001.