Table 3.
Mechanosensitive miRNAs inhibit osteogenic differentiation.
| miRNAs | Mechanical Stimuli | Methods | Target Gene | Samples | Reference |
|---|---|---|---|---|---|
| miR-154-5p | Stretch stress (Four-point bending) |
2000 με, 0.5 Hz 2 h/day |
Wnt 11 | ADSCs | [37] |
| miR-103a | Stretch stress (Flexcell) |
8% elongation, 0.5 Hz, 72 h | Runx2 | hFOB1.19 | [23] |
| miR-195-5p | Stretch stress (Flexcell) |
12% deformation 0.1 Hz, 72 h |
WNT3A BMPR1A FGF2 |
PDLCs | [54] |
| miR-503-5p | Stretch stress (Flexcell) |
10% elongation, 1 Hz, 12 h | - | BMSCs | [22] |
| miR-29 family (a,b,c) | Stretch stress (Flexcell) Compressive force |
12% deformation, 0.1 Hz, 24 h, 2 g/cm2, 24 h | Col1a1 Col3a1 Col5a1 | PDLCs | [55] |
| miR-138 | Shockwave (ESW) |
0.16 mJ/mm2, 500 impulses, 10 min | FAK | MSCs | [57] |
| miR-132-3p | Unloading | 30 rpm, 48 h | Ep300 | primary osteoblast | [60] |
ADSCs: adipose-derived mesenchymal stem cells; BMPR1A: bone morphogenetic protein receptor-1A; BMSCs: Bone marrow mesenchymal stem cells; Col I: Collage I; Ep300: E1A binding protein p300; ESW: Extracorporeal shockwave; FAK: Focal adhesion kinase; FGF2: fibroblast growth factor 2; FSS: Fluid shear stress; Hmga2: high mobility group AT-hook 2; miR: microRNA; MSCs: mesenchymal stem cells; PDLCs: periodontal ligament cells; Runx2: runt related transcription factor 2; WNT3A: WNT family member 3A. WNT11: WNT family member 11.