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. 2017 Aug 3;18(8):1688. doi: 10.3390/ijms18081688

Figure 3.

Figure 3

Specificity of the ZHER2:2891-Fc binding. (a) Fluorescent microscopy images of the HER2-positive SK-BR-3 and MDA-MB-231 HER2-negative cells. The anti-HER2 antibody was detected with a FITC-labeled secondary antibody whereas the affibody was FITC labelled, scale bar = 100 µm; (b) ZHER2:2891-Fc binding to the SK-BR-3 and MDA-MB-231 cells analyzed by Western blotting. The upper panel shows HER2 levels in SK-BR-3 and MDA-MB-231cells, the middle panel severs as a loading control for the experiment, the bottom panel demonstrates that ZHER2:2891-Fc is specifically enriched at the surface of the SK-BR-3 cells.