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Conjugation of MMAE to ZHER2:2891-Fc. (a) The ZHER2:2891-Fc species before and after conjugation separated on 12% SDS-PAGE gel and subjected to Coomassie staining. The samples were heated (96 °C, 10 min) in non-reducing Laemmli buffer prior the electrophoretic separation; (b) The mass spectrum of the conjugation product; (c) The ZHER2:2891-Fc-MMAE conjugate migration on a native 12% polyacrylamide gel under non-reducing conditions stained with Coomassie [45].
