Figure 8.

Replication kinetics of CHIKV and DENV-2 in mono or dually infected MG and SG. Viral RNA copy number was determined by RT-qPCR at the indicated times post-virus exposure. (A,B) CHIKV RNA was determined in MG (A) and SG (B) of mosquitoes mono-infected by CHIKV (●) or co-infected by CHIKV and DENV-2 (■), respectively); (C,D) DENV-2 RNA was determined in MG (A) and SG (B) of mosquitoes mono-infected by DENV-2 (●) or co-infected by DENV-2 and CHIKV (■). Eight mosquitoes were dissected at each time point and three replicates were performed. * indicates statistical differences between mon-infected and co-infected organs.