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. 2017 Aug 7;18(8):1724. doi: 10.3390/ijms18081724

Figure 4.

Figure 4

Morphology of human induced pluripotent stem cells (hiPSC) after hepatic differentiation over 17 days in different media and/or in co-culture with human umbilical vein endothelial cells (HUVEC). The pictures show hiPSC after hepatic differentiation in mono-culture over 17 days in hepatocyte culture medium (HCM-I/II) (A), in a 1:1 mixture of hepatocyte culture medium and endothelial cell growth medium EGM, consisting of basal medium and supplements (HCM-I/II + EGM complete) (B) or in HCM enriched with EGM supplements (HCM-I/II + EGM supplements) (C); hiPSC after hepatic differentiation in co-culture with HUVEC on day 7 of differentiation using HCM-I/II + EGM complete (D) or HCM-I/II + EGM supplements (E); hiPSC after hepatic differentiation in co-culture with HUVEC on day 17 of differentiation using HCM-I/II + EGM complete (F) or HCM-I/II + EGM supplements (G). HUVEC were growing in free spaces between the hiPSC (arrows). Scale bars correspond to 300 µm.