Figure 7.

Effect of different media compositions and/or co-culture with human umbilical vein endothelial cells (HUVEC) on secretion of stage-specific markers during differentiation of human induced pluripotent stem cells (hiPSC). Differentiation of definitive endodermal cells was performed over 14 days using hepatocyte culture medium (HCM-I/II), a 1:1 mixture of hepatocyte culture medium and endothelial cell growth medium, consisting of basal medium and supplements (HCM-I/II + EGM complete) or HCM enriched with endothelial cell growth supplements (HCM-I/II + EGM supplements) with or without HUVEC addition. Graphs show the secretion of α-fetoprotein (AFP, A), secretion of albumin (B) and secretion of urea (C). The area under the curve was calculated and differences between HCM and all other groups as well as differences between test media and their corresponding co-cultures were detected using the unpaired, two-tailed Student’s t-test, n = 8; Co-cultures: n = 3; mean ± standard error of the mean.