Table 2.
Culture media and culture medium combinations used for testing in cultures of human umbilical vein endothelial cells (HUVEC) or human induced pluripotent stem cells (hiPSC).
| Component | EGM complete | HCM-I | HCM-II | HCM-I/II + EGM Complete | HCM-I/II + EGM Supplements |
|---|---|---|---|---|---|
| Hepatocyte culture medium (HCM) Bullet Kit | - | 100% (v/v) | 50% (v/v) | 97.5% (v/v) | |
| Endothelial cell growth medium (EGM) | 97.5% (v/v) | - | 48.75% (v/v) | - | |
| EGM Supplements | 2.5% (v/v) | - | 1.25% (v/v) | 2.5% (v/v) | |
| Human hepatocyte growth factor, recombinant | - | 10 ng/mL | 10 ng/mL | 10 ng/mL | 10 ng/mL |
| Human oncostatin M, recombinant | - | - | 10 ng/mL | 10 ng/mL 1 | 10 ng/mL 1 |
| Gentamycin | 0.05 mg/mL | 0.05 mg/mL | 0.05 mg/mL | 0.05 mg/mL | 0.05 mg/mL |
1 only added to media containing HCM-II.