Table 4.
Primary and secondary antibodies used for immunofluorescence staining.
| Antibody Type and Specifity | Protein Symbol | Species | Manufacturer | Article-No. | Final Conc. (µg/mL) |
|---|---|---|---|---|---|
| Primary Antibody | |||||
| Cytokeratin 18 | CK18 | mouse | Santa Cruz | Sc-6259 | 2 |
| Hepatocyte nuclear factor 4 α | HNF4A | rabbit | Santa Cruz | Sc-8987 | 4 |
| Platelet endothelial cell adhesion molecule 1 | PECAM1 | mouse | Abcam | ab24590 | 5 |
| POU domain, class 5, transcription factor 1 | OCT3 | rabbit | Santa Cruz | Sc-9081 | 2 |
| Von Willebrand factor | VWF | rabbit | Abcam | ab6994 | 35.5 |
| Secondary antibody | |||||
| Alexa Fluor® 488 anti-mouse | goat | Life Technologies | A-11029 | 2 | |
| Alexa Fluor® 594 anti-rabbit | goat | Life Technologies | A-11037 | 2 |