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. 2017 Aug 11;18(8):1747. doi: 10.3390/ijms18081747

Figure 2.

Figure 2

Nucleotide sequence of the promoter region (position 1 to 1460 bp upstream from the start codon), of the MT gene of Biomphalaria glabrata. Metal responsive elements (MREs) acting as potential binding sites for the presumed metal transcription factor I are highlighted in lilac. Potential transcription factor binding sites (TFBs) involved in stress response are marked in orange and hypothetical binding sites for regulatory transcription factors are marked in green. The putative TATA boxes are highlighted in blue and the start codon of the first exon is shaded in grey. Functional specification of TFBs identified using TRANSFAC: metal induction (lilac): MREs: aatccgtGTGCAaatt, tggccgtGTGCAtata, tggccgtGTGCAta (black line indicates palindromic MRE sequence of the MRE in opposite direction). Stress response (orange): HSF: GTTCT, AGAAC, agaaaattcaACTCG, AGAAAagaaaattca, TGAAGagaatatgcg; C/EBPγ: acaATTTCaaaat; PPARγ: tatCCTTTagccaca; YAP: aTTAGTaa, aAGCAT, agcaggATACGtaatgtatt. Transcriptional regulation (green): AP-1: atgAGTCAc, tgaTGAGTcacat; ATF: gaaacaCGTCAa, gaataaCGTCAc; Cdx-1: TTTATt, ttctTTTATgtccatg, TTTATt, aATAAA; cMyc: gaccATGTGgtg; GATA-1: ttTTATCagc, ttTTATCtct, gccctTATCAtttt; MEF-2C: atAAATA, TATTTtt; POU2F1: aTTTGCatt; POU1F1a: aattATTCAt; p300: cgGTAGT, ACGTTcg; PBF: tctCTTTTgtt, CCTTTt; TBP: tTTTATgtcca, tTTTATcagct. For abbreviations and functional explanation of the single TFBs, see Table 1.