Table 2.
Accession No. a | Protein Name | Plant Species b | Coverage (%) c | Peptides (95%) d | iTRAQ Ratio | Expression Pattern e |
---|---|---|---|---|---|---|
20 °C vs 5 °C | ||||||
gi|462406120 | hypothetical protein PRUPE_ppa001062mg | Prunus persica | 40.28 | 53 | 1.47 | ↑ |
gi|462409482 | hypothetical protein PRUPE_ppa002222mg | Prunus persica | 45.78 | 47 | 1.58 | ↑ |
gi|462403952 | hypothetical protein PRUPE_ppa003093mg | Prunus persica | 48.76 | 38 | 1.54 | ↑ |
gi|462415344 | hypothetical protein PRUPE_ppa000130mg | Prunus persica | 19.75 | 33 | 0.74 | ↓ |
gi|462400695 | hypothetical protein PRUPE_ppa004306mg | Prunus persica | 50.10 | 43 | 1.32 | ↑ |
gi|392055980 | plasma membrane H+-ATPase | Malus baccata var. xiaojinensis | 27.88 | 22 | 0.40 | ↓ |
gi|462419972 | hypothetical protein PRUPE_ppa005369mg | Prunus persica | 53.23 | 29 | 1.63 | ↑ |
gi|428697281 | photosystem II CP43 chlorophyll apoprotein | Fragaria virginiana | 34.88 | 39 | 0.59 | ↓ |
gi|462411072 | hypothetical protein PRUPE_ppa000855mg | Prunus persica | 29.05 | 26 | 2.09 | ↑ |
gi|462403979 | hypothetical protein PRUPE_ppa002220mg | Prunus persica | 28.76 | 22 | 0.36 | ↓ |
gi|462418213 | hypothetical protein PRUPE_ppa000841mg | Prunus persica | 15.04 | 14 | 0.69 | ↓ |
gi|6136112 | UTP—glucose-1-phosphate uridylyltransferase; | Pyrus pyrifolia | 37.79 | 14 | 1.66 | ↑ |
gi|462395206 | hypothetical protein PRUPE_ppa004819mg | Prunus persica | 36.73 | 15 | 1.64 | ↑ |
gi|568246711 | photosystem II protein D1 | Pyrus spinosa | 40.23 | 21 | 0.53 | ↓ |
gi|462407840 | hypothetical protein PRUPE_ppa010884mg | Prunus persica | 40.95 | 28 | 3.50 | ↑ |
gi|568246731 | photosystem I P700 apoprotein A1 | Pyrus spinosa | 16.40 | 14 | 0.39 | ↓ |
gi|110083901 | dehydroascorbate reductase | Malus domestica | 53.99 | 14 | 2.13 | ↑ |
gi|2981475 | putative cinnamyl alcohol dehydrogenase | Malus domestica | 34.46 | 11 | 1.41 | ↑ |
gi|462404058 | hypothetical protein PRUPE_ppa000235mg | Prunus persica | 7.75 | 10 | 0.54 | ↓ |
gi|462401406 | hypothetical protein PRUPE_ppa010331mg | Prunus persica | 43.87 | 18 | 0.52 | ↓ |
gi|462402851 | hypothetical protein PRUPE_ppa002111mg | Prunus persica | 23.22 | 13 | 2.33 | ↑ |
gi|462419292 | hypothetical protein PRUPE_ppa009763mg | Prunus persica | 27.24 | 13 | 0.47 | ↓ |
gi|568246730 | photosystem I P700 apoprotein A2 | Pyrus spinosa | 18.53 | 15 | 0.42 | ↓ |
gi|302399085 | TCP domain class transcription factor | Malus domestica | 16.67 | 7 | 0.31 | ↓ |
gi|462419362 | hypothetical protein PRUPE_ppa004904mg | Prunus persica | 18.52 | 7 | 0.49 | ↓ |
gi|462408065 | hypothetical protein PRUPE_ppa012896mg | Prunus persica | 47.33 | 10 | 1.71 | ↑ |
gi|118136427 | vacuolar H+-PPase | Malus domestica | 6.85 | 4 | 0.33 | ↓ |
gi|357528260 | granule-bound starch synthase GBSS1 | Malus domestica | 28.34 | 19 | 3.02 | ↑ |
gi|470135580 | PREDICTED: 60S ribosomal protein L3-like | Fragaria vesca subsp. vesca | 13.11 | 6 | 0.10 | ↓ |
gi|470137537 | PREDICTED: coproporphyrinogen-III oxidase, chloroplastic-like | Fragaria vesca subsp. vesca | 9.07 | 3 | 0.69 | ↓ |
gi|462419678 | hypothetical protein PRUPE_ppa004988mg | Prunus persica | 7.05 | 3 | 0.19 | ↓ |
gi|421953224 | BIS1 biphenyl synthase | Malus domestica | 11.79 | 5 | 0.12 | ↓ |
gi|377829931 | rps7 gene product (chloroplast) | Pentactina rupicola | 23.23 | 5 | 6.31 | ↑ |
gi|462400922 | hypothetical protein PRUPE_ppa006524mg | Prunus persica | 7.84 | 3 | 0.19 | ↓ |
gi|462415015 | hypothetical protein PRUPE_ppa012878mg | Prunus persica | 20.53 | 3 | 0.21 | ↓ |
gi|462422777 | hypothetical protein PRUPE_ppa011659mg | Prunus persica | 12.38 | 2 | 0.67 | ↓ |
gi|470101387 | PREDICTED: 60S ribosomal protein L5-like | Fragaria vesca subsp. vesca | 7.69 | 2 | 0.32 | ↓ |
gi|462406042 | hypothetical protein PRUPE_ppa002386mg | Prunus persica | 3.10 | 2 | 8.17 | ↑ |
gi|462407732 | hypothetical protein PRUPE_ppa010094mg | Prunus persica | 15.53 | 5 | 0.17 | ↓ |
gi|470127406 | PREDICTED: 40S ribosomal protein S6-like | Fragaria vesca subsp. vesca | 9.64 | 2 | 0.09 | ↓ |
gi|462418868 | hypothetical protein PRUPE_ppa000009mg | Prunus persica | 0.28 | 1 | 0.28 | ↓ |
0 °C vs 20 °C | ||||||
gi|462416702 | hypothetical protein PRUPE_ppa001865mg | Prunus persica | 52.93 | 68 | 0.48 | ↓ |
gi|462396213 | hypothetical protein PRUPE_ppa025698mg | Prunus persica | 34.58 | 31 | 0.65 | ↓ |
gi|462400695 | hypothetical protein PRUPE_ppa004306mg | Prunus persica | 50.10 | 43 | 0.73 | ↓ |
gi|568246744 | cytochrome f | Pyrus spinosa | 62.81 | 31 | 0.59 | ↓ |
gi|428697281 | photosystem II CP43 chlorophyll apoprotein (chloroplast) | Fragaria virginiana | 34.88 | 39 | 0.56 | ↓ |
gi|470128485 | PREDICTED: phosphoribulokinase, chloroplastic-like | Fragaria vesca subsp. vesca | 51.96 | 37 | 0.39 | ↓ |
gi|568246756 | photosystem II CP47 chlorophyll apoprotein | Pyrus spinosa | 29.72 | 32 | 0.65 | ↓ |
gi|462407840 | hypothetical protein PRUPE_ppa010884mg | Prunus persica | 40.95 | 28 | 0.45 | ↓ |
gi|381393060 | glyceraldehyde-3-phosphate dehydrogenase A | Pyrus × bretschneideri | 51.12 | 58 | 0.28 | ↓ |
gi|6177796 | JPR ORF1 | Pyrus pyrifolia | 18.16 | 10 | 0.48 | ↓ |
gi|302399085 | TCP domain class transcription factor | Malus domestica | 16.67 | 7 | 3.13 | ↑ |
gi|470135580 | PREDICTED: 60S ribosomal protein L3-like | Fragaria vesca subsp. vesca | 13.11 | 6 | 4.79 | ↑ |
gi|462400607 | hypothetical protein PRUPE_ppa002173mg | Prunus persica | 9.49 | 6 | 0.58 | ↓ |
gi|421953224 | BIS1 biphenyl synthase | Malus domestica | 11.79 | 5 | 3.47 | ↑ |
gi|462400922 | hypothetical protein PRUPE_ppa006524mg | Prunus persica | 7.84 | 3 | 2.96 | ↑ |
gi|470101387 | PREDICTED: 60S ribosomal protein L5-like | Fragaria vesca subsp. vesca | 7.69 | 2 | 1.80 | ↑ |
gi|462405695 | hypothetical protein PRUPE_ppa004869mg | Prunus persica | 3.89 | 2 | 1.69 | ↑ |
gi|470103122 | probable cadmium/zinc-transporting ATPase HMA1, chloroplastic-like | Fragaria vesca subsp. vesca | 1.72 | 2 | 7.11 | ↑ |
0 °C vs 5 °C | ||||||
gi|462415344 | hypothetical protein PRUPE_ppa000130mg | Prunus persica | 19.75 | 33 | 0.58 | ↓ |
gi|568246744 | cytochrome f | Pyrus spinosa | 62.81 | 31 | 0.55 | ↓ |
gi|4165550 | apgm | Malus domestica | 47.76 | 27 | 1.43 | ↑ |
gi|428697281 | photosystem II CP43 chlorophyll apoprotein (chloroplast) | Fragaria virginiana | 34.88 | 39 | 0.33 | ↓ |
gi|462411072 | hypothetical protein PRUPE_ppa000855mg | Prunus persica | 29.05 | 26 | 2.11 | ↑ |
gi|462403979 | hypothetical protein PRUPE_ppa002220mg | Prunus persica | 28.76 | 22 | 0.52 | ↓ |
gi|470128485 | PREDICTED: phosphoribulokinase, chloroplastic-like | Fragaria vesca subsp. vesca | 51.96 | 37 | 0.54 | ↓ |
gi|462408129 | hypothetical protein PRUPE_ppa006653mg | Prunus persica | 59.35 | 30 | 0.59 | ↓ |
gi|568246756 | photosystem II CP47 chlorophyll apoprotein | Pyrus spinosa | 29.72 | 32 | 0.54 | ↓ |
gi|568246711 | photosystem II protein D1 | Pyrus spinosa | 40.23 | 21 | 0.35 | ↓ |
gi|568246731 | photosystem I P700 apoprotein A1 | Pyrus spinosa | 16.40 | 14 | 0.23 | ↓ |
gi|110083901 | dehydroascorbate reductase | Malus domestica | 53.99 | 14 | 2.09 | ↑ |
gi|381393060 | glyceraldehyde-3-phosphate dehydrogenase A | Pyrus × bretschneideri | 51.12 | 58 | 0.34 | ↓ |
gi|2981475 | putative cinnamyl alcohol dehydrogenase | Malus domestica | 34.46 | 11 | 1.32 | ↑ |
gi|462402866 | hypothetical protein PRUPE_ppa000990mg | Prunus persica | 12.03 | 11 | 0.43 | ↓ |
gi|462419292 | hypothetical protein PRUPE_ppa009763mg | Prunus persica | 27.24 | 13 | 0.48 | ↓ |
gi|568246730 | photosystem I P700 apoprotein A2 | Pyrus spinosa | 18.53 | 15 | 0.41 | ↓ |
gi|462395764 | hypothetical protein PRUPE_ppa008222mg | Prunus persica | 35.88 | 12 | 0.69 | ↓ |
gi|568246726 | photosystem II protein D2 | Pyrus spinosa | 24.93 | 23 | 0.22 | ↓ |
gi|313600351 | beta-1,3-glucanase | Malus hupehensis | 24.28 | 11 | 2.00 | ↑ |
gi|462422251 | hypothetical protein PRUPE_ppa001168mg | Prunus persica | 8.54 | 7 | 1.91 | ↑ |
gi|118136427 | vacuolar H+-PPase | Malus domestica | 6.85 | 4 | 0.52 | ↓ |
gi|462405098 | hypothetical protein PRUPE_ppa008755mg | Prunus persica | 14.69 | 8 | 0.41 | ↓ |
gi|470135580 | PREDICTED: 60S ribosomal protein L3-like | Fragaria vesca subsp. vesca | 13.11 | 6 | 0.49 | ↓ |
gi|470135491 | PREDICTED: signal recognition particle 54 kDa protein, chloroplastic-like | Fragaria vesca subsp. vesca | 11.78 | 6 | 2.91 | ↑ |
gi|470137537 | PREDICTED: coproporphyrinogen-III oxidase, chloroplastic-like | Fragaria vesca subsp. vesca | 9.07 | 3 | 0.64 | ↓ |
gi|462419678 | hypothetical protein PRUPE_ppa004988mg | Prunus persica | 7.05 | 3 | 0.42 | ↓ |
gi|421953224 | BIS1 biphenyl synthase | Malus domestica | 11.79 | 5 | 0.46 | ↓ |
gi|470101387 | PREDICTED: 60S ribosomal protein L5-like | Fragaria vesca subsp. vesca | 7.69 | 2 | 0.58 | ↓ |
gi|462406042 | hypothetical protein PRUPE_ppa002386mg | Prunus persica | 3.10 | 2 | 3.05 | ↑ |
gi|462407696 | hypothetical protein PRUPE_ppa004527mg | Prunus persica | 5.54 | 2 | 7.80 | ↑ |
gi|462405695 | hypothetical protein PRUPE_ppa004869mg | Prunus persica | 3.89 | 2 | 2.07 | ↑ |
gi|470127406 | PREDICTED: 40S ribosomal protein S6-like | Fragaria vesca subsp. vesca | 9.64 | 2 | 0.15 | ↓ |
gi|462424295 | hypothetical protein PRUPE_ppa000786mg | Prunus persica | 1.79 | 2 | 0.60 | ↓ |
20 °C + Ca vs 20 °C | ||||||
gi|462403952 | hypothetical protein PRUPE_ppa003093mg | Prunus persica | 48.76 | 38 | 0.51 | ↓ |
gi|462403979 | hypothetical protein PRUPE_ppa002220mg | Prunus persica | 28.76 | 22 | 0.41 | ↓ |
gi|2981475 | putative cinnamyl alcohol dehydrogenase | Malus domestica | 34.46 | 11 | 0.66 | ↓ |
gi|462402851 | hypothetical protein PRUPE_ppa002111mg | Prunus persica | 23.22 | 13 | 0.41 | ↓ |
gi|313600351 | beta-1,3-glucanase | Malus hupehensis | 24.28 | 11 | 0.39 | ↓ |
gi|114795078 | glutathione S-transferase | Pyrus communis | 24.51 | 5 | 0.60 | ↓ |
gi|421953224 | BIS1 biphenyl synthase | Malus domestica | 11.79 | 5 | 2.47 | ↑ |
gi|470129084 | PREDICTED: GTP-binding protein SAR1A-like | Fragaria vesca subsp. vesca | 11.40 | 3 | 1.79 | ↑ |
gi|470103122 | probable cadmium/zinc-transporting ATPase HMA1, chloroplastic-like | Fragaria vesca subsp. vesca | 1.72 | 2 | 8.09 | ↑ |
0 °C + Ca vs 0 °C | ||||||
gi|462403979 | hypothetical protein PRUPE_ppa002220mg | Prunus persica | 28.76 | 22 | 0.10 | ↓ |
gi|462418975 | hypothetical protein PRUPE_ppa003053mg | Prunus persica | 32.07 | 20 | 1.61 | ↑ |
gi|462405014 | hypothetical protein PRUPE_ppa007588mg | Prunus persica | 33.15 | 13 | 1.64 | ↑ |
gi|462402866 | hypothetical protein PRUPE_ppa000990mg | Prunus persica | 12.03 | 11 | 2.36 | ↑ |
gi|313600351 | beta-1,3-glucanase | Malus hupehensis | 24.28 | 11 | 0.39 | ↓ |
gi|462422598 | hypothetical protein PRUPE_ppa001487mg | Prunus persica | 16.32 | 12 | 0.56 | ↓ |
gi|462405098 | hypothetical protein PRUPE_ppa008755mg | Prunus persica | 14.69 | 8 | 2.38 | ↑ |
gi|357528260 | granule-bound starch synthase GBSS1 | Malus domestica | 28.34 | 19 | 1.96 | ↑ |
gi|470135580 | PREDICTED: 60S ribosomal protein L3-like | Fragaria vesca subsp. vesca | 13.11 | 6 | 0.14 | ↓ |
gi|462409757 | hypothetical protein PRUPE_ppa003798mg | Prunus persica | 7.30 | 5 | 5.11 | ↑ |
gi|46093878 | sucrose phosphate phosphatase | Malus domestica | 13.18 | 5 | 0.76 | ↓ |
gi|462401293 | hypothetical protein PRUPE_ppa009351mg | Prunus persica | 19.59 | 5 | 0.21 | ↓ |
gi|470137537 | PREDICTED: coproporphyrinogen-III oxidase, chloroplastic-like | Fragaria vesca subsp. vesca | 9.07 | 3 | 1.41 | ↑ |
gi|470125921 | PREDICTED: pantothenate kinase 2-like | Fragaria vesca subsp. vesca | 6.72 | 6 | 1.38 | ↑ |
gi|470127406 | PREDICTED: 40S ribosomal protein S6-like | Fragaria vesca subsp. vesca | 9.64 | 2 | 0.75 | ↓ |
a The protein ID from National Center for Biotechnology Information (NCBI); b The plant species that matched the peptides. Accession number was recorded as a reference for the identification in NCBI_Rosaceae database, and the species origin annotation of all matching proteins were belonging to the Rosaceae; c The amino acid sequence coverage for the identified proteins; d The number of distinct peptide sequences in the protein group; e ↑ upregulated, ↓ downregulated.