Figure 4.

AI activates adiponectin-AMPK mediated signaling pathways in mice. (A) Plasma adiponectin concentrations; (B) representative western blot analysis and quantification of AdipoR2, total adenosine monophosphate-activated protein kinase (AMPK), p-AMPK, total S6K1, and p-S6K1 in the liver; Representative reverse transcription polymerase chain reaction (RT-PCR) product bands and quantitative comparison of mRNA expression for (C) lipogenesis, (D) lipolysis, fatty acid oxidation, inflammation and (E) triglyceride synthesis in the liver. Results are presented as the mean ± SEM. Statistical analysis was performed using one-way ANOVA and analyzed further by Duncan’s multiple range test. Different letters above the bars indicate significant differences among experimental groups (p < 0.05).