Table 1.

Differentially-expressed protein spots identified from the two-dimensional gel electrophoresis of Rosa hybrida ‘Rock Fire’ leaf proteome.

Spot No. a	Accession Number b	Nominal Mass (Mr) c	Theo./Exp. pI d	Protein Identification	Species	Sc (%) e	Score f
Photosynthesis
7	Q332S1	45577	5.43/5.47	NAD(P)H-quinone oxidoreductase subunit H	Lactuca sativa	84	40
24	A6MML0	18820	6.19/5.05	NAD(P)H-quinone oxidoreductase subunit J	Dioscorea elephantipes	39	47
12	Q7F8E8	40407	8.72/4.45	Ferredoxin-NADP reductase	Oryza sativa Japonica	18	20
10	Q945L0	9476	5.33/5.10	Cytochrome c oxidase subunit 6b-2	Arabidopsis thaliana	60	46
21	A9L9A7	9.59/5.40	9.59/5.20	Photosystem I assembly protein Ycf4	Lemna minor	36	40
28	A9L9A7	21440	9.59/6.15	Photosystem I assembly protein Ycf4	Lemna minor	36	40
23	P19312	20087	7.60/6.50	Ribulose bisphosphate carboxylase small chain SSU5B	Lemna gibba	28	34
26	P21218	43587	9.23/4.50	Protochlorophyllide reductase B	Arabidopsis thaliana	25	30
30	A7M975	21497	9.59/4.10	Photosystem I assembly protein Ycf4	Cuscuta reflexa	28	36
Energy metabolism
9	Q7XKV5	60290	7.21/5.90	β-glucosidase 11	Oryza sativa Japonica	19	35
15	Q9SCV4	94293	8.09/5.10	β -galactosidase 8	Arabidopsis thaliana	9	41
3	Q9SE26	64295	7.28/4.00	Isocitrate lyase	Dendrobium crumenatum	20	40
25	P12300	56038	6.61/6.70	Glucose-1-phosphate adenylyltransferase large subunit	Triticum aestivum	16	32
27	Q8H2J9	47104	9.76/6.80	Glycerol-3-phosphate dehydrogenase (NAD+)	Oryza sativa Japonica	22	35
11	P85438	3396	9.99/4.10	Acetyl-CoA carboxylase	Catharanthus roseus	100	36
16	P85438	3398	9.99/5.10	Acetyl-CoA carboxylase	Catharanthus roseus	96	34
22	Q9LHH7	31589	5.77/6.55	Bifunctional protein FolD 2	Arabidopsis thaliana	38	51
Transcription/translation
20	A4QK54	13682	9.39/4.55	50S ribosomal protein L14	Arabis hirsuta	54	38
32	Q9FF52	17946	9.02/5.15	60S ribosomal protein L12-3	Arabidopsis thaliana	51	34
40	B5X0I6	124486	5.20/4.45	Protein CTR9 homolog	Arabidopsis thaliana	15	36
5	Q94JQ4	19930	8.53/5.25	Reactive Intermediate Deaminase A	Arabidopsis thaliana	66	38
39	Q43317	34492	6.25/5.20	Cysteine synthase	Citrullus lanatus	40	40
36	Q5NTH4	33310	9.30/4.30	Shikimate kinase 1	Oryza sativa Japonica	34	45
14	A2YMU2	29709	9.35/5.10	Ribosome-recycling factor,	Oryza sativa Indica Group	23	33
17	Q32RJ9	49484	9.55/5.87	tRNA(Ile)-lysidine synthase	Zygnema circumcarinatum	13	32
Stress/redox homeostasis
8	P23283	21940	8.91/4.00	Desiccation-related protein PCC3-06	Craterostigma plantagineum	38	33
18	Q08298	42658	9.42/4.60	Dehydration-responsive protein RD22	Elaeis guineensis var. tenera	20	28
37	P01053	9381	6.58/6.15	Subtilisin-chymotrypsin inhibitor-2A	Hordeum vulgare	38	27
19	Q40679	27454	6.00/4.00	Peroxygenase	Oryza sativa Indica	25	29
13	P30109	24081	5.54/6.80	Glutathione S-transferase PARB	Nicotiana tabacum	41	36
Ion binding
2	Q9ZPX9	15339	4.34/6.80	Calcium-binding protein KIC	Arabidopsis thaliana	28	19
6	B6TB21	28001	5.20/4.45	Anamorsin homolog	Zea mays	48	30
31	O24475	72088	5.48/4.00	Pinene synthase	Abies grandis	14	28
33	Q0IMG5	8379	5.62/5.60	Metallothionein-like protein 4A	Oryza sativa Japonica	69	29
34	P29390	27863	5.75/4.31	Ferritin-2	Zea mays	23	36
Ubiquitination
4	Q9FZ48	17277	6.74/4.47	Ubiquitin-conjugating enzyme E2 8	Arabidopsis thaliana	59	32
38	P35131	17277	6.74/4.60	Ubiquitin-conjugating enzyme E2 36	Arabidopsis thaliana	59	32
35	Q8GY23	404995	4.96/4.45	E3 ubiquitin-protein ligase	Arabidopsis thaliana	17	34
Putative
1	Q9FKQ2	13622	8.53/5.38	Putative clathrin assembly protein	Pinus koraiensis	57	35
29	Q2R075	43258	6.62/5.55	Putative glutaredoxin-C11	Oryza sativa Indica	24	34

^a The spot no. corresponds to the numbers given in protein gel images (Figure 8); ^b Protein accession number determined by SwissProt database via the MASCOT software [33]; ^c Theoretical molecular mass (Mr) calculated from MASCOT Peptide Mass Fingerprint; ^d Isoelectric point (pI) of spots identified from MASCOT Peptide Mass Fingerprint and protein gel images (Figure 8); ^e MASCOT score of protein hit; ^f Sequence coverage percentage.