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. 2017 Aug 16;18(8):1779. doi: 10.3390/ijms18081779

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of genetic stability of ASCs cultured in DMEM/FBS and STK2. To examine the genetic stability of ASCs, cells were cultured in the presence or absence of mitomycin C (mit-C), and were subjected to CBMN assay as described in the Methods section. Mit-C was used as the positive control. (A) Binucleated cells were analyzed for the presence of micronuclei (MN), nucleoplasmic bridges (NPB), and nuclear buds (NBUD). Images were captured at 400× (scale bar, 10 μm); (B) Each frequency was evaluated and presented as a bar graph (n = 4). Data represent the mean ± SD of the average of at least three independent experiments. * vs. DMEM/FBS (P9). * p < 0.001.