Figure 7.

The BpWOX genes respond to the environmental stresses. (a) The expression pattern of BpWOX gene under four environmental conditions. For cold treatment, the seedlings were transferred into a 4 °C growth chamber. For salt and CdCl₂ treatments, the seedlings were washed carefully and transferred into a solution of 200 mM NaCl and 50 µM CdCl₂, respectively. For drought treatment, we used 20% (w/v) PEG solution to simulate drought condition. BpWOX2 was labeled with a red line, BpWOX3 was labeled with a blue line, BpWOX4 was labeled with a yellow line, BpWOX8 was labeled with a green line, BpWOX9 was labeled with a purple line, and BpWOX10 was labeled with a pink line; (b) A heat map of BpWOX genes responding to phytohormones at the transcription level. For phytohormone treatments, 100 µM solution of IAA, GA, SA, Me-JA, ETH, and ABA were sprayed onto the surface of the seedlings, respectively. Transcript levels were determined by qRT-PCR, and the expression of BpGAPDH was used as an internal control.