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. 2017 Aug 17;18(8):1783. doi: 10.3390/ijms18081783

Figure 2.

Figure 2

Analysis of apoptosis in mouse brain microvascular endothelial cells (MBMEC) exposed to different doses of fullerenols under basal and inflammatory conditions. (A) Representative Western blot analyses of full-length caspase 3 protein expression in MBMEC cultures after exposure to interferon-γ and tumor necrosis factor-α (I + T; 100 IU each) and treatment with fullerenols (F; 1, 10 and 100 µg/mL; n = 3) for 18 h compared to cultures under basal conditions. Staurosporine treatment (Stauro; 1 μM for 2 h) was used as a positive control; (B) Densitometric quantification of the amount of full-length caspase 3 in MBMEC, treated with different doses of fullerenols or vehicle (normalized to untreated control cells) cultured for 18 h under basal conditions; (C) Densitometric quantification of the amount of full-length caspase 3 in MBMEC, treated with different doses of fullerenols or vehicle (normalized to untreated control cells) cultured for 18 h under inflammatory conditions; (D) Representative immunofluorescence images of cleaved caspase 3 (red) counterstained with Hoechst (blue) in MBMEC treated with fullerenols (F 1, 1 µg/mL; F 10, 10 µg/mL; F 100, 100 µg/mL) for 18 h under basal or inflamed (I + T; 100 IU each) conditions. Staurosporine treatment (1 μM for 2 h) was used as a positive control. Scale bar counts for all immunofluorescence images. Ctrl, untreated control; Veh, vehicle treatment.