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Macroscopic pictures and morphology of human eschar tissue. (a) Eschar tissue on patient; (b) Eschar after tangential excision; (c) Eschar at interface of non-viable and viable tissue from which acellular Burn Wound Extract (BWE) is derived (see Materials and Methods); hematoxylin and eosin staining showing the absence of epidermis; small, rounded cells are present in the lower levels of the dermis (areas with blue nuclei; see arrows). Scale bar = 1 cm (a,b) or 200 µm (c).
