Figure 4.

The suppressive effect of miR-30a-5p on the proliferation and migration of UTUC cells. BFTC-909 cells were transfected with miR-30a-5p mimetic agent, followed by WST-1 and trans-well assays. NTC denotes non-transfection controls with scrambled mimetic treatment. (A) After mimetic transfection for 48 h, the WST-1 assay was used to evaluate cell numbers. The WST-1 value is directly proportional to cell number; (B) After mimetic transfection for 72 h, trans-well migration assay was performed to evaluate the cell migration ability; (C) The crystal violet-stained cells are those penetrating the trans-well membrane. All data are shown as means ± SEM from three independent experiments. * indicates that p < 0.001 between the indicated groups.