Figure 3.

Confocal fluorescence micrographs of PC3 cells incubated for (a) 30 min and (b) 72 h on bare Si substrate; (c) Composite and (d) DAPI-only micrographs of PC3 cells incubated for 72 h on small C-shaped pillars (group 1). Pillar locations are indicated by solid arrows while bulged edges of the DAPI-stained materials are highlighted by dashed arrows in (c). A schematic drawing of the focal plane used in these micrographs is shown in (e). Representative micrographs of PC3 cells incubated for 72 h reveal fine line structures emanating from the small C-shaped pillars are displayed in (f–h). Lines in (f) are connected with the adjacent pillars while lines in (g) and (h) are not. Confocal micrographs of 72 h incubated PC3 cells on large C-shaped and hollow-shaped pillars are displayed in (i,j), respectively. Inset drawings indicate the pillar orientations.