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(a) DAPI only micrograph revealing fine line structures in cells incubated on small C-shaped pillars. High magnification images of a representative nucleus surrounding two small C-shaped pillars imaged in various focal planes are shown in (b–e). The corresponding focal plane locations are illustrated in the schematic drawing. Orthogonal views of this nucleus are shown in (f) revealing two pillars and a nano-slit. Red triangle pointers indicate substrate surface locations.
