Figure 5.

Recruitment of kinesin-1 reverses direction of pigment granule transport induced by pigment aggregation signals. Phase contrast images of a cell co-expressing kinesin-1-EGFP- FRB and TRP-1-FKBP-mCherry treated with melatonin to aggregate pigment granules before (left) or after (right) treatment with rapalog; rapalog-induced recruitment of kinesin-1 leads to dispersion of pigment granules, and therefore reverses direction of their dynein-dependent movement. Numbers indicate time in minutes. Scale bar, 5 μm.