Skip to main content
. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Jun 29;1863(9):2333–2341. doi: 10.1016/j.bbadis.2017.06.021

Figure 3. TAI-heightened miR-34a-5p down-regulates Bdnf through targeting its 3′UTR.

Figure 3

(A, B) miR-34a-5p inhibits the expression of Bdnf by targeting the 3′UTR of predicted binding sites at nucleotides 2020–2044 and 2427–2449 of Bdnf 3′UTR. The generated mutant sites at the Bdnf 3′UTR seed region are indicated. The wild-type Bdnf 3′UTR (or mutant) was inserted into the downstream of luciferase reporter gene immediately in the pGL3-control vector. (C, D) The effect of miR-34a-5p (or anti-miR-34a-5p) on the pGL3-Bndf-2427 and pGL3-Bndf-2020 reporters in 3T3 cells were measured by luciferase reporter assays. (E, F) The effect of miR-34a-5p (or anti-miR-34a-5p) on the expression of Bdnf in 3T3 cells was measured by Western blotting. Each experiment was repeated at least three times. Statistically significant differences are indicated: **P < 0.01; ***P < 0.005; Student t-test.