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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Jun 29;1863(9):2333–2341. doi: 10.1016/j.bbadis.2017.06.021

Figure 6. Administration of miR-34a-5p antagomir intravenous retains the intestinal bacterial community after TAI.

Figure 6

(A, B) Principal component (A) and β diversity (B) analyses were used to measure the shift of intestinal bacterial flora profile in TAI exposure mice after radiation at day 3 and day 6, n = 5. Statistically significant differences are indicated: Wilcoxon rank sum test. (C, D) Principal component (C) and β diversity (D) analyses were used to measure the shift of intestinal bacterial flora profile in mice treated with miR-34a-5p antagomir following TAI at day 3 and day 6, n = 5. Statistically significant differences are indicated: Wilcoxon rank sum test. (E) The difference of intestinal bacterial structure at phylum level of mice on the day before TAI, day 3 and day 6 after TAI was examined using 16S high throughput sequencing, n = 5. (F) Tail intravenous injection of miR-34a-5p antagomir was performed immediately following TAI. The difference of intestinal bacterial structure at phylum level of mice on the day before TAI, day 3 and day 6 after TAI was examined using 16S high throughput sequencing, n = 5. (G) The mRNA level of MyD88 in small intestine tissues was assessed by qRT-PCR. The samples were obtained from mice without TAI, with TAI or treated with miR-34a-5p antagomir following TAI individually. **P < 0.01; Student t-test, n = 12. (H) The escape latency time to reach the hidden platform during the 5 days of Morris water maze test was assessed. F (1, 47) = 3.443 between control and FMT with TAI-exposed donors group, F (1, 47) = 0.993 between FMT from TAI-exposed donors and FMT from TAI-exposed with miR-34a-5p antagomir injection group, P > 0.05, ANOVA, n = 12. (I) The times spent in target quadrant and swimming traces in 60 s at day 6 of Morris water maze were shown. F (1, 11) = 9.262 between control and FMT with TAI-exposed donors group, F (1, 11) = 4.073 between FMT from TAI-exposed donors and FMT from TAI-exposed donors with miR-34a-5p antagomir injection group, *P < 0.05, ANOVA, n = 12.