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. Author manuscript; available in PMC: 2017 Aug 31.
Published in final edited form as: Nat Commun. 2014;5:3301. doi: 10.1038/ncomms4301

Figure 1. Human genome-wide siRNA ionomics screen.

Figure 1

(a) Effect of siRNA on copper levels in HeLa cells. Cells were transfected with human siATP7A, and 72 h after transfection, copper was analyzed by ICP-MS. Error bars are one fold standard variances calculated from three replicates. (b) Impact of ATP7A deficiency on copper levels in HeLa cells. Cells were subjected to ATP7A knockdown as in (a) and examined by X-ray fluorescence microscopy. Scrambled RNA was used as control. Signals of copper and phosphorus are shown. The same single cell is shown in left and right panels. (c) siRNA ionomics screen. HeLa cells were transfected with the Human Genome SMARTpool Dharmacon siRNA library in 96-well plates followed by analysis of trace elements by ICP-MS. Concentrations of elements were analyzed to obtain normalized z-scores for each gene from the library and select primary hits.