qPCR analysis of Jag1-null bones. Gene expression of Notch pathway components (A) Jag1, (B) Hes1 and (C) Hey1, osteogenic genes (D) osteocalcin (Ocn), (E) osterix (Osx) and (F) collagen type-I (Col1a1) and pro-osteoclastogenesis genes (G) receptor activator of nuclear factor kappa-B ligand (RANKL), (H) osteoprotegerin (Opg) and (I) tartrate-resistant acid phosphatase (TRAP). For all analyses, data are presented as fold change expression to each genotype's respective whole bone (Prx1-Cre;Jag1f/f or Col2.3-Cre;Jag1f/f) calculated using the formula 2−ΔΔC(t) (*p < 0.050, #p < 0.100).