Figure 4.

(A–E) Expressions of ICAM-1 and E-selectin in immunohistochemistry (IHC) stained clinical EVLP donor lung tissue and association with perfusate IL-1β levels. (A) Paraffin-embedded donor lung sections DAB stained for ICAM-1 expression. Arrows mark the low-intensity stained (i) or high-intensity stained (ii) pulmonary vascular endothelium. Unselective background staining seen on adhesive alveolar epithelium. Box and whisker plots show average ICAM-1 (B) and E-selectin (D) staining intensity scores for pre-EVLP and post-EVLP biopsy specimens from 4 Declined lungs and 4 Survival lungs. Scatter plots show the correlation between average ICAM-1 (C) and E-selectin (E) intensity scores in the same experiment and corresponding perfusate IL-1β levels (linear regression line with 95% confidence intervals). *p < 0.05, ***p < 0.001.