Table 1. Oligonucleotides used in this study.
Primer | Sequence 5' → 3' |
---|---|
NEO-RT-F | AGACAATCGGCTGCTCTGAT |
NEO-RT-R | CTCGTCCTGCAGTTCATTCA |
Actin-RT-F | TGGCACCATACCTTCTACAACGAG |
Actin-RT-R | CGTCATCTTCTCACGGTTCTGC |
5UTR-Lin07.0150-F | ATCAGCTACAACCCGTGTCC |
5UTR-Lin31.1630-F | CTACCTTCTTGACCTTCGCG |
NEO::DHFR-F | TCGCCTTCTTGACGAGTTCT |
DHFR::NEO-R | TAGCCGAATAGCCTCTCCAC |
RNA-Control | UCCUGCUUCAACAGUGCUUGGACGGAAC-Biotin |
NEO-end-F | GCATCGCCTTCTATCGCCTT |
3UTR-07.0150-R | CGGCTCATTCTAGCAGCTCA |
3UTR-31.1630-R | GCAAACGTGTCCACTGTCGA |