Skip to main content
. 2017 Aug 31;12(8):e0183988. doi: 10.1371/journal.pone.0183988

Fig 2. CPE activated PI3K-Akt-AS160 signaling in C2C12 cells.

Fig 2

C2C12 cells were exposed to CPE (50 or 100 μg/mL) or insulin (100 nM) for 30 min. The phosphorylation level of AS160 at Thr642 (A), Akt at Ser473 and Thr308 (B), and effects of CPE on the 2NBDG uptake in C2C12 cells treated with 50 μM Perifosine, Akt inhibitor (C). IRS1 and p85 subunit of PI3K complex (D) was determined by immunoprecipitation. Data are expressed as the mean ± SEM of three independent experiments. **p < 0.01 vs. the vehicle-treated control.