Skip to main content
. 2017 Aug 31;12(8):e0184182. doi: 10.1371/journal.pone.0184182

Fig 2. IGFBP2 overexpression enhances adipogenic differentiation in WJCMSCs.

Fig 2

(A) Flag-IGFBP2-infected WJCMSCs showed IGFBP2 overexpression by real-time RT-PCR. GAPDH was used as an internal control. (B) Overexpression of IGFBP2 was verified by Western Blot analysis. (C-D) Oil Red O staining and quantitative analysis showed that IGFBP2 overexpression prompted formation of lipid deposits. Scale bar: 100 μm. (E-H) Real-time RT-PCR showed that overexpression of IGFBP2 upregulated expressions of PPARγ (E), LPL (F), CD36 (G), and CEBPA (H) in WJCMSCs at 0, 1, and 2 weeks after induction. GAPDH was used as an internal control. *p < 0.05. **p < 0.01. α: anti; w: week.