Fig 3. IGFBP2 activates JNK and Akt signaling pathways.

(A) Western Blot analysis demonstrated that IGFBP2 overexpression caused an increase in p-JNK and p-Akt in WJCMSCs, however, the total amounts of JNK, ERK, p38, and Akt proteins were not affected; The phosphorylated p38 protein was not found. (B) Quantitative analysis of p-ERK, p-JNK, and p-Akt based on Western Blot results for the WJCMSC-Flag-IGFBP2 cells and WJCMSC-Vector cells. Total ERK, JNK, and Akt were used as internal control respectively. **p < 0.01. α: anti.