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. 2017 Aug 31;12(8):e0184182. doi: 10.1371/journal.pone.0184182

Fig 5. Effect of Akt inhibitor on IGFBP2-induced adipogenic differentiation of WJCMSCs.

Fig 5

(A) Western Blotting results showed a reduction of p-Akt in WJCMSC-Flag-IGFBP2 following treatment with an Akt inhibitor, LY294002 (10 μM, 20 μM, 50 μM or 80 μM in DMSO) for 1 h during adipogenic induction. (B) Quantitative analysis of p-Akt based on Western Blot results. Total Akt was used as internal control. The expression levels that are indicated with the same letter do not differ significantly. (C-D) Oil Red O staining and quantitative analysis showed that 10 μM LY294002 effectively inhibited IGFBP2-mediated adipogenic differentiation. Scale bar: 100 μm. (E-F) Real-time RT-PCR results showed downregulated expressions of PPARγ (E) and LPL (F) in WJCMSC-Flag-IGFBP2 cells following 10 μM LY294002 treatment during adipogenic induction at 1, 2, and 3 weeks. GAPDH was used as an internal control. **p < 0.01. α: anti; w: week.