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. 2017 Aug 31;12(8):e0184182. doi: 10.1371/journal.pone.0184182

Fig 6. IGFBP2-mediated Akt activation is abrogated by JNK inhibitor.

Fig 6

(A) Western Blot results indicated that administration of 20 μM SP600125 decreased JNK activation and abrogated Akt phosphorylation in WJCMSC-Flag-IGFBP2 cells. (B) Quantitative analysis of p-JNK and p-Akt based on Western Blot results for the WJCMSC-Vector cells, WJCMSC-Flag-IGFBP2 cells, and WJCMSC-Flag-IGFBP2 + 20 μM SP600125 cells. Total Akt and JNK were used as internal control respectively. (C) Western Blotting results showed administration of 10 μM LY294002 decreased the level of p-Akt activation, while had no effect on JNK phosphorylation in WJCMSC-Flag-IGFBP2 cells. (D) Quantitative analysis of p-Akt and p-JNK based on Western Blot results. Total Akt and JNK were used as internal control respectively. **p < 0.01. α: anti.