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. 2017 Aug 31;12(8):e0181034. doi: 10.1371/journal.pone.0181034

Fig 2. Effects of the Wnt5a-long (L) and Wnt5a-short (S) mRNA isoforms on cell proliferation and spheroid formation of colorectal cancer (CRC) cells.

Fig 2

(A) Validation of expression levels of the different Wnt5a mRNA isoforms after transfection with specific siRNAs of the different Wnt5a isoforms for 48 h in the HCT116 cell line. (B) The proliferative ability of HCT116 cells after transfection with specific siRNAs of different Wnt5a isoforms. (C) HCT116 cells were transfected with specific siRNAs of different Wnt5a isoforms, and foci were visualized after crystal violet staining. (D) The combined results of all transfections, wherein total focal counts ± SD are illustrated. (E) HCT116 cells were grown in 3D NanoCulture Plates (NCPs) with transfection with specific siRNAs of different Wnt5a isoforms. In the 3D-NCP condition, siWnt5a-L isoform-transfected spheroid cells appeared to increase in spheroid size, but siWnt5a-S isoform-transfected spheroid cells appeared to spread out from the spheroids, and the spheroid sizes were reduced.