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. 2017 Aug 31;12(8):e0181034. doi: 10.1371/journal.pone.0181034

Fig 3. siWnt5a-short (S) isoform-induced apoptotic death in HCT116 cells.

Fig 3

(A) HCT116 cells were transfected with specific siRNAs of different Wnt5a isoforms for 48 h. Cells were subjected to an annexin-V assay using a Muse cell analyzer. The method led to four different populations of cells: live cells (annexin-V negative (lower right quadrant)), early apoptotic cells (positive for annexin-V (lower right corner)), late apoptotic/dead cells (annexin V positive (upper right quadrant)), and non-apoptotic dead cells (upper left quadrant). (B) Quantification of total apoptotic cells (early apoptosis + late apoptotic cells) determined by annexin-V positivity. (C) HCT116 cells transfected with specific siRNAs of different Wnt5a isoforms for 48 h, subjected to a Western blot analysis, and probed for CDK4, caspase-3, poly(ADP ribose) polymerase (PARP), and β-catenin antibodies, respectively. β-Actin was used as the loading control.