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. 2017 Aug 31;12(8):e0181034. doi: 10.1371/journal.pone.0181034

Fig 7. Knockdown of the Wnt5a-short (S) mRNA isoform enhanced the sensitivity of colorectal cancer (CRC) cells to 5-azacytidine (5-Aza).

Fig 7

(A) The Wnt5a-S mRNA isoform was silenced for 48 h with 100 nM of specific siRNA, following which cells were treated with 5-Aza at the indicated concentrations for an additional 48 h. Cells were stained with annexin V and analyzed using a Muse Cell Analyzer. (B) The quantification result of annexin V-positive cells with knockdown of the Wnt5a-S isoform alone or in the presence of 5-Aza. (C) Western blot analysis of DNMT1, caspase-3, poly (ADP ribose) polymerase (PARP) cleavage, and β-catenin in HCT116 cells transfected with control siRNA or Wnt5a-S isoform-specific siRNA oligomers for 48 h and subsequently incubated with 5-Aza at the indicated concentrations for an additional 48 h. Arrow symbol indicated that siWnt5a-S isoform knockdown HCT116 cells in the present with 5-Aza showed more significant at inducing caspase-3 cleavage than control siRNA-transfected HCT116 cells in the presence of 5-Aza. β-actin is shown as the loading control.